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er t2 creer t2 sequence  (Addgene inc)


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    Addgene inc er t2 creer t2 sequence
    (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused <t>CreER</t> is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.
    Er T2 Creer T2 Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/er t2 creer t2 sequence/product/Addgene inc
    Average 95 stars, based on 93 article reviews
    er t2 creer t2 sequence - by Bioz Stars, 2026-02
    95/100 stars

    Images

    1) Product Images from "CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages"

    Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages

    Journal: bioRxiv

    doi: 10.1101/2025.05.23.655316

    (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused CreER is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.
    Figure Legend Snippet: (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused CreER is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.

    Techniques Used: Translocation Assay, Negative Control, Expressing, Marker, Comparison

    (A) Confocal images of liver cryosections from 2-month post-fertilization (mpf) Tg(fabp10a:BB-NTR) zebrafish expressing CreER , ERCreER , or DDCreER , without any 4-hydroxytamoxifen (4-OHT) or trimethoprim (TMP) treatment. Fluorescent markers indicate non-recombined nls-mTagBFP2+, or recombined H2B-mGL+ or mCherry-NTR+ hepatocytes. Scale bar: 20 µm. (B) Bar plot representing Mean ± SD showing quantification of background recombination at 2 mpf based on histological analysis of liver sections from Tg(fabp10a:NTR) zebrafish. Significance determined by two-way ANOVA followed by Tukey’s multiple comparisons test. (C) Flow cytometry-based quantification of background recombination at 3 mpf in Tg(fabp10a:BB-NTR) zebrafish. The proportion of mTagBFP2+, H2B-mGL+, mCherry+, and double-positive (H2B-mGL+/mCherry+) hepatocytes was measured by FACS. Each dot represents one animal and the bar plots represent Mean ± SD. Statistical analysis performed using two-way ANOVA followed by Tukey’s multiple comparisons test.
    Figure Legend Snippet: (A) Confocal images of liver cryosections from 2-month post-fertilization (mpf) Tg(fabp10a:BB-NTR) zebrafish expressing CreER , ERCreER , or DDCreER , without any 4-hydroxytamoxifen (4-OHT) or trimethoprim (TMP) treatment. Fluorescent markers indicate non-recombined nls-mTagBFP2+, or recombined H2B-mGL+ or mCherry-NTR+ hepatocytes. Scale bar: 20 µm. (B) Bar plot representing Mean ± SD showing quantification of background recombination at 2 mpf based on histological analysis of liver sections from Tg(fabp10a:NTR) zebrafish. Significance determined by two-way ANOVA followed by Tukey’s multiple comparisons test. (C) Flow cytometry-based quantification of background recombination at 3 mpf in Tg(fabp10a:BB-NTR) zebrafish. The proportion of mTagBFP2+, H2B-mGL+, mCherry+, and double-positive (H2B-mGL+/mCherry+) hepatocytes was measured by FACS. Each dot represents one animal and the bar plots represent Mean ± SD. Statistical analysis performed using two-way ANOVA followed by Tukey’s multiple comparisons test.

    Techniques Used: Expressing, Flow Cytometry



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    (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused <t>CreER</t> is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.
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    Image Search Results


    (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused CreER is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.

    Journal: bioRxiv

    Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages

    doi: 10.1101/2025.05.23.655316

    Figure Lengend Snippet: (A) Schematic overview of the DDCreER system. In the absence of both 4-hydroxytamoxifen (4-OHT) and trimethoprim (TMP), the destabilizing domain (DD) fused CreER is targeted for proteasomal degradation. Addition of TMP alone stabilizes the DD-fused CreER, resembling the conventional CreER system and potentially allowing background recombination. Addition of 4-OHT alone promotes nuclear translocation of DDCreER, but only of the residual protein that has not yet been degraded, permitting low-level recombination. Only in the presence of both 4-OHT and TMP is DDCreER stabilized and translocated to the nucleus, enabling efficient recombination. Created in BioRender. Gurzov, E. (2025) https://BioRender.com/uteh37g (B) Confocal images of livers from 10 days post-fertilization (dpf) Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) zebrafish larvae following treatment with 172 µM TMP, 10 µM 4-OHT, or both together. DMSO (1%) served as a negative control. Fluorescent markers indicate non-recombined cells (mTagBFP2+), and recombined hepatocytes (H2B-mGL+ or mCherry-NTR+). Scale bar: 50 µm. (C) Quantification of recombination efficiency in Tg(fabp10a:BB-NTR); Tg(fabp10a:DDCreER) larvae under different treatments. Bar plots show the percentage of hepatocytes expressing each fluorescent marker (mTagBFP2, H2B-mGL, or mCherry). Data represents Mean ± SD. Statistical significance was calculated using two-way ANOVA followed by Tukey’s multiple comparisons test. (D) Comparison of recombination efficiency between the DDCreER and ERCreER systems, based on the percentage of BFP+ hepatocytes. Bar plots depict Mean ± SD. Statistical significance was assessed using ordinary one-way ANOVA followed by Tukey’s multiple comparisons test. Significantly lower proportions of unrecombined (BFP+) cells were observed in DDCreER-expressing livers.

    Article Snippet: The ER T2 CreER T2 sequence was obtained fom pCAG-ER T2 CreER T2 , which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) .

    Techniques: Translocation Assay, Negative Control, Expressing, Marker, Comparison

    (A) Confocal images of liver cryosections from 2-month post-fertilization (mpf) Tg(fabp10a:BB-NTR) zebrafish expressing CreER , ERCreER , or DDCreER , without any 4-hydroxytamoxifen (4-OHT) or trimethoprim (TMP) treatment. Fluorescent markers indicate non-recombined nls-mTagBFP2+, or recombined H2B-mGL+ or mCherry-NTR+ hepatocytes. Scale bar: 20 µm. (B) Bar plot representing Mean ± SD showing quantification of background recombination at 2 mpf based on histological analysis of liver sections from Tg(fabp10a:NTR) zebrafish. Significance determined by two-way ANOVA followed by Tukey’s multiple comparisons test. (C) Flow cytometry-based quantification of background recombination at 3 mpf in Tg(fabp10a:BB-NTR) zebrafish. The proportion of mTagBFP2+, H2B-mGL+, mCherry+, and double-positive (H2B-mGL+/mCherry+) hepatocytes was measured by FACS. Each dot represents one animal and the bar plots represent Mean ± SD. Statistical analysis performed using two-way ANOVA followed by Tukey’s multiple comparisons test.

    Journal: bioRxiv

    Article Title: CellCousin2: An Optimized System for Partial Ablation and Tracing of Regenerative Lineages

    doi: 10.1101/2025.05.23.655316

    Figure Lengend Snippet: (A) Confocal images of liver cryosections from 2-month post-fertilization (mpf) Tg(fabp10a:BB-NTR) zebrafish expressing CreER , ERCreER , or DDCreER , without any 4-hydroxytamoxifen (4-OHT) or trimethoprim (TMP) treatment. Fluorescent markers indicate non-recombined nls-mTagBFP2+, or recombined H2B-mGL+ or mCherry-NTR+ hepatocytes. Scale bar: 20 µm. (B) Bar plot representing Mean ± SD showing quantification of background recombination at 2 mpf based on histological analysis of liver sections from Tg(fabp10a:NTR) zebrafish. Significance determined by two-way ANOVA followed by Tukey’s multiple comparisons test. (C) Flow cytometry-based quantification of background recombination at 3 mpf in Tg(fabp10a:BB-NTR) zebrafish. The proportion of mTagBFP2+, H2B-mGL+, mCherry+, and double-positive (H2B-mGL+/mCherry+) hepatocytes was measured by FACS. Each dot represents one animal and the bar plots represent Mean ± SD. Statistical analysis performed using two-way ANOVA followed by Tukey’s multiple comparisons test.

    Article Snippet: The ER T2 CreER T2 sequence was obtained fom pCAG-ER T2 CreER T2 , which was a gift from Connie Cepko (Addgene plasmid # 13777 ; http://n2t.net/addgene:13777 ; RRID:Addgene_13777) .

    Techniques: Expressing, Flow Cytometry